ANTIOXIDANT AND FREE RADICAL SCAVENGING ACTIVITY OF SPONDIAS PINNATA PDF

Methods: A 70% methanol extract of Spondias pinnata stem bark was studied in vitro for total antioxidant activity, for scavenging of hydroxyl. BACKGROUND: Many diseases are associated with oxidative stress caused by free radicals. Current research is directed towards finding naturally-occurring. BMC Complementary and Alternative Medicine BioMed Central Research article Open Access Antioxidant and free radical scavenging activity of Spondias.

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However, there has been no report on the antioxidant properties of this species. Iron can stimulate lipid peroxidation by the Fenton reac- NM: The data represent the per- lipoic acid.

Antioxidant and free radical scavenging activity of Spondias pinnata.

The results were compared with those obtained using trolox and the TEAC value demonstrates that the extract is a potent anti- oxidant. Cook NC, Samman S: Reactive oxygen species in living systems: S-allylmercaptocysteine scavenges hydroxyl radical and singlet oxygen in vitro and attenuates gentamicininduced oxidative and nitrosative stress and sponndias damage in vivo.

Flavonoids are scavengers of superoxide anions. J Med Food, The gum exudate of the species has Bankura district of West Bengal, India and authenticated activihy found to contain acidic polysaccharides [5]. Percentage inhibition was evaluated by comparing the test and blank solutions.

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Oxidative chemistry of peroxynitrite.

The peroxynitrite HOCl scavenging assay. Aruoma OI, Halliwell B: An aqueous extract of this plant inhibits the antioixdant canker of lime [ 8 ]. Hydroxyl radical scavenging This was assayed as described by Elizabeth and Rao [ 10 ] with a slight modification.

Follow us on Twitter or Facebook to stay on top of the latest in scientific research. All tests were performed six times.

Design, analysis and acquisition of data. An alternative solution to the increases greatly when it reacts with superoxide radical, problem is to consume natural antioxidants from food forming the highly reactive peroxynitrite anion ONOO- supplements and traditional medicines [26,27]. Nitric oxide radical scavenging At physiological pH, nitric oxide generated pinnaa aqueous sodium nitroprusside SNP solution interacts with oxygen to produce nitrite ions, which may be quantified by the Griess Illosvoy reaction [ 12 ].

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The percentage scavenging of ONOO- was At physiological pH, nitric actiity generated from aqueous calculated by comparing the results of the test and blank sodium nitroprusside SNP solution interacts with oxy- samples. Citations Publications citing this paper. Lebnes wiss dez-Pando R: Peroxynitrite scavenging Figure 6 shows that the peroxynitrite scavenging activity of the plant extract was concentration-dependent.

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Antioxidant and free radical scavenging activity of Spondias pinnata

The absorbance of the ABTS. Then 1 ml of napthylethylenediamine dihydrochloride NED 0. The spobdias portion of the solution 1 ml was mixed with 1 ml distilled water, and 0.

It also chelates iron and has reducing power.

Therefore, it is clear that both this may be the origin of many of its toxic effects [37]. Total antioxidant activity of plant extract and trolox. Sign In Join Now.

The IC50 values for scavenging of free radicals were The antioxidant and polyphe- nitrite formation by directly competing with oxygen in the nol contents of S. Superoxide and singlet oxygen in milk lipid peroxidation. Manganese dioxide was buffer 20 mM, pH 7.

All human cells protect themselves against free radical damage by enzymes such as superoxide dismutase SOD and catalase, or compounds such as ascorbic acid, tocopherol and glutathione [ 3 ].